Rabbit anti-Cow IgG (Whole Molecule) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN117037

Quick Overview for Rabbit anti-Cow IgG (Whole Molecule) Antibody (Biotin) - Preadsorbed (ABIN117037)

Target: IgG

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Cow

Host: Rabbit

Characteristics: Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.

Purification: Preadsorption: Solid phase absorption

Immunogen: Bovine IgG whole molecule

Application Details

Application Notes: ImmunoblottingELISAImmunohistochemistryImmunomicroscopyas well as other antibody based assays using streptavidin or avidin conjugates requiringlot-to-lot consistency. This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISAusing Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) sodium azide as preservative. N-Hydroxysuccinimide Ester (BAC).

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store vial at 2-8 °C short term only.

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Bovine Immunoglobulin G